|
KMID : 0613820190290020147
|
|
Journal of Life Science
2019 Volume.29 No. 2 p.147 ~ p.151
|
|
|
Breeding of a Recessive Soybean Genotype (titirs2rs2) with Green Cotyledons and Black Seed Coats
|
|
Choi Sang-Woo
Kim Jin-A
Shim Sang-In
Kim Min-Chul
Chung Jong-Il
|
|
|
Abstract
|
|
|
|
Soybean [Glycine max (L.) Merr.] is grown worldwide for its high protein and oil content. Anthocyanins from black soybean seed coats are known to have many pharmaceutical effects. Soybean cultivars with large seed sizes and black seed coats are needed by soybean farmers. However, antinutritional factors, like protein, stachyose, and Kunitz trypsin inhibitor (KTI) exist in raw mature soybeans. Genetic elimination or reduction of these components is needed in soybean breeding. The objective of this research was to develop new a soybean strain with black seed coats and green cotyledons that was KTI protein free and low in stachyose. Six parents were used. The presence or absence of KTI protein was detected using the Western blot technique. The content of stachyose in mature seeds was detected using HPLC. One new strain was selected from 11 F2 plants with black seed coats and green cotyledons that lacked KTI protein. The new strain had black seed coats and green cotyledons and was KTI protein free and low in stachyose. The plant height of the new strain was 66 cm, and its 100-seed weight was 28.4 g. The stachyose content of the new strain was 2.59 g/kg. The new strain developed in this research will be used to develop new cultivars that are KTI protein free and low in stachyose.
|
|
|
KEYWORD
|
|
|
Black soybean, Kunitz trypsin inhibitor (KTI), new strain, stachyose
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|